Abstract: This article mainly studies the effect of Fe content on the microstructure and mechanical mroperty of TC4 titanium alloy. Comparative analysis was conducted on the room temperature tension, 400℃ high temperature tension, room temperature impact, and metallographic microstructure of TC4 sample 1 with 0.05% Fe added and TC4 sample 2 with 0.23% Fe added after the same forging and heat treatment. The results indicate that increasing the Fe content（about 0.2%, mass fraction） is beneficial for primary growth α phase content decreased by about 9%. And secondary α phase thickness increases and they are arranged in a chaotic and intersecting manner. The strength of room temperature stretching, room temperature impact, and 400℃ high temperature stretching is significantly improved. The tensile strength and yield strength at room temperature increased by approximately 55MPa. The tensile strength increased by about 30MPa for 400℃ high-temperature stretching. This is because during the stretching process, the obstacles encountered during the slip of dislocations are enhanced, resulting in a large number of dislocations accumulating in the dispersed strengthening phase; At the same time, with the increase of Fe element,significant lattice distortion occurs when forming a solid solution with titanium alloy, which can also improve the strength of titanium alloy. But it has no significant effect on the plasticity of room temperature stretching and 400℃high-temperature stretching.