Abstract: Laminated gradient nano-twinned copper LNT-Cu（0/50/0） was prepared using deformation-assisted jet electrodeposition with the variable load of 0, 50, 0N. The cross-sectional morphology and grain microstructure of LNT-Cu（0/50/0） were characterized by field emission scanning electron microscopy（SEM）. The results demonstrated that the laminatedgradient nano-twinned copper produced by this method is a dense, defect-free sample. The grain structure of LNT-Cu（0/50/0） transitions from columnar grains at the bottom to equiaxed grains in the middle, and back to columnar grains at the top, with average grain sizes of 550nm, 157nm, and 531nm, respectively. The crosssectional microhardness and tensile properties of LNT-Cu（0/50/0）, as well as those of DAJED-Cu-0N and DAJEDCu-50N, were tested using a Vickers microhardness tester and an electronic universal testing machine. The fracture morphology of LNT-Cu（0/50/0）was observed using SEM. The results showed that the cross-sectional hardness of LNT-Cu（0/50/0） exhibited a laminated gradient distribution, with an average microhardness of 150HV0.2 in the 0N deposition region and up to 190HV0.2 in the 50N deposition region. The cross-sectional microhardness in each region closely matched that of the corresponding single-load samples. The fracture morphology indicated ductile fracture characterized by microvoid coalescence. The yield strength of LNT-Cu（0/50/0） reached 339MPa, with a uniform elongation of 3.7%, showing a balanced mechanical performance.